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Engineering    2017, Vol. 3 Issue (1) : 55-59     https://doi.org/10.1016/J.ENG.2017.01.007
Research |
趋化信号接枝的掺镁羟基磷灰石促进间充质干细胞归巢
Pistone Alessandro1(),Iannazzo Daniela1,Espro Claudia1,Galvagno Signorino1,Tampieri Anna2,Montesi Monica2,Panseri Silvia2,Sandri Monica2
1. Department of Engineering, University of Messina, Messina 98166, Italy
2. Institute of Science and Technology for Ceramics, National Research Council of Italy, Faenza 48018, Italy
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摘要 

干细胞归巢,即间充质干细胞定向趋化募集至损伤处,对体内骨再生起重要作用。本文以人纤连蛋白片段III1-C(FF III1-C) 及纤连蛋白类似物的肽序列Gly-Arg-Gly-Asp-Ser-Pro-Lys 作为趋化因子,分别共价结合至掺镁羟基磷灰石中,用于研究模拟肽序列接枝的掺镁羟基磷灰石对间充质细胞归巢的调控作用。用于检测间充质干细胞活力的MTT 法初步研究发现释放趋化信号的掺镁羟基磷灰石可有效促进干细胞迁移。

关键词 掺镁羟基磷灰石间充质干细胞趋化/ 趋触因子骨组织工程    
Abstract

Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

Keywords Mg-doped hydroxyapatite      Mesenchymal stem cells      Chemotactic/haptotactic factors      Bone tissue engineering     
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通讯作者: Pistone Alessandro     E-mail: pistone@unime.it
最新录用日期:    在线预览日期:    发布日期: 2017-03-02
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Alessandro Pistone
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引用本文:   
Alessandro Pistone,Daniela Iannazzo,Claudia Espro, et al. Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite[J]. Engineering, 2017, 3(1): 55-59.
网址:  
http://engineering.org.cn/EN/10.1016/J.ENG.2017.01.007     OR     http://engineering.org.cn/EN/Y2017/V3/I1/55
Fig.1  Scheme of the synthesis of MgHA-APTES disks. RT: room temperature.
Fig.2  XRD spectra of MgHA and MgHA-APTES disks.
Fig.3  SEM images of (a) MgHA and (b) MgHA-APTES samples.
Fig.4  TEM images of (a) MgHA and (b) MgHA-APTES samples.
Fig.5  Scheme of the synthesis of MgHA-APTES-CFP and MgHA-APTES-CFF. Reagents and conditions: Gly-Arg-Gly-Asp-Ser-Pro-Lys or FF III1-C, EDC×HCl, NHS, PBS, 3 h, 37 °C.
Fig.6  TGA profiles of MgHA, MgHA-APTES, MgHA-APTES-CFP100, and MgHA-APTES-CFF100 samples.
Fig.7  FTIR spectra of MgHA, MgHA-APTES, and MgHA-APTES-CFF100.
Fig.8  MTT test of mMSC, 3 days after the seeding into the inserts placed in direct contact with the disks. Cells-only group used as a control.
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