Please wait a minute...
Submit  |   Chinese  | 
 
Advanced Search
   Home  |  Online Now  |  Current Issue  |  Focus  |  Archive  |  For Authors  |  Journal Information   Open Access  
Submit  |   Chinese  | 
Engineering    2017, Vol. 3 Issue (1) : 130 -135     https://doi.org/10.1016/J.ENG.2017.01.001
Research |
Quality Monitoring of Porous Zein Scaffolds: A Novel Biomaterial
Yue Zhang,Wei-Ying Li,Run Lan,Jin-Ye Wang()
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract
Abstract  

Our previous studies have shown that zein has good biocompatibility and good mechanical properties. The first product from a porous scaffold of zein, a resorbable bone substitute, has passed the biological evaluation of medical devices (ISO 10993) by the China Food and Drug Administration. However, Class III medical devices need quality monitoring before being placed on the market, and such monitoring includes quality control of raw materials, choice of sterilization method, and evaluation of biocompatibility. In this paper, we investigated four sources of zein through amino acid analysis (AAA) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in order to monitor the composition and purity, and control the quality of raw materials. We studied the effect of three kinds of sterilization method on a porous zein scaffold by SDS-PAGE. We also compared the changes in SDS-PAGE patterns when irradiated with different doses of gamma radiation. We found that polymerization or breakage did not occur on peptide chains of zein during gamma-ray (γ-ray) sterilization in the range of 20–30 kGy, which suggested that γ-ray sterilization is suitable for porous zein scaffolds. Regarding cell compatibility, we found a difference between using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and a cell-counting kit-8 (CCK-8) assay to assess cell proliferation on zein film, and concluded that the CCK-8 assay is more suitable, due to its low background optical density.

Keywords Zein      Amino acid analysis      SDS-PAGE      Gamma-ray sterilization      MTT assay      CCK-8 assay     
Fund: 
Corresponding Authors: Jin-Ye Wang   
Just Accepted Date: 20 January 2017   Online First Date: 23 January 2017    Issue Date: 02 March 2017
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
Yue Zhang
Wei-Ying Li
Run Lan
Jin-Ye Wang
Cite this article:   
Yue Zhang,Wei-Ying Li,Run Lan, et al. Quality Monitoring of Porous Zein Scaffolds: A Novel Biomaterial[J]. Engineering, 2017, 3(1): 130 -135 .
URL:  
http://engineering.org.cn/EN/10.1016/J.ENG.2017.01.001     OR     http://engineering.org.cn/EN/Y2017/V3/I1/130
References
1   Gianazza E, Viglienghi V, Righetti PG, Salamini F, Soave C. Amino acid composition of zein molecular components. Phytochemistry 1977;16(3):315–7
doi: 10.1016/0031-9422(77)80054-X
2   Shukal R, Munir C. Zein: the industrial protein from corn. Ind Crop Prod 2001;13(3):171–92
doi: 10.1016/S0926-6690(00)00064-9
3   Dong J, Sun Q, Wang J. Basic study of corn protein, zein, as a biomaterial in tissue engineering, surface morphology and biocompatibility. Biomaterials 2004;25(19):4691–7
doi: 10.1016/j.biomaterials.2003.10.084 pmid: 15120515
4   Gong S, Wang H, Sun Q, Xue S, Wang J. Mechanical properties and in vitro biocompatibility of porous zein scaffolds. Biomaterials 2006;27(20):3793–9
doi: 10.1016/j.biomaterials.2006.02.019 pmid: 16527348
5   Wang H, Gong S, Lin Z, Fu J, Xue S, Huang J, et al. In vivo biocompatibility and mechanical properties of porous zein scaffolds. Biomaterials 2007;28(27):3952–64
doi: 10.1016/j.biomaterials.2007.05.017 pmid: 17582490
6   Wang J, Wang H, Gong S. Preparation of zein scaffold for tissue engineering. In: Proceedings of the 8th World Biomaterials Congress ; 2008 May 28–Jun 1; Amsterdam, the Netherlands. North Miami Beach: Curran Associates, Inc.; 2008. p. 4:1787.
7   Phillip E Jr, Murthy NS, Bolikal D, Narayanan P, Kohn J, Lavelle L, et al. Ethylene oxide’s role as a reactive agent during sterilization: effects of polymer composition and device architecture. J Biomed Mater Res-A 2013;101(4):532–40
doi: 10.1002/jbm.b.32853 pmid: 23296710
8   Lee MH, Kim HL, Kim CH, Lee SH, Kim JK, Lee SJ,et al. Effects of low temperature hydrogen peroxide gas on sterilization and cytocompatibility of porous poly(D, L-lactic-co-glycolic acid) scaffolds. Surf Coat Tech 2008;202(22– 23):5762–7
doi: 10.1016/j.surfcoat.2008.06.114
9   Faraj KA, Brouwer KM, Geutjes PJ, Versteeg EM, Wismans RG, Deprest JA, et al. The effect of ethylene oxide sterilisation, beta irradiation and gamma irradiation on collagen fibril-based scaffolds. Tissue Eng Regen Med 2011;8(5):460–70.
10   Rnjak-Kovacina J, DesRochers TM, Burke KA, Kaplan DL. The effect of sterilization on silk fibroin biomaterial properties. Macromol Biosci 2015;15(6):861–74
doi: 10.1002/mabi.201500013 pmid: 25761231
11   Zhang Z, Chen W. Plating densities, alpha-difluoromethylornithine effects and time dependence on the proliferation of IEC-6 cells. China Med J 2002;115(4):518–20
pmid: 12133287
12   Cetin Y, Bullerman LB. Evaluation of reduced toxicity of zearalenone by extrusion processing as measured by the MTT cell proliferation assay. J Agr Food Chem 2005;53(16):6558–63
doi: 10.1021/jf051120z pmid: 16076149
13   Bruggisser R, von Daeniken K, Jundt G, Schaffner W, Tullberg-Reinert H. Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Med 2002;68(5):445–8
doi: 10.1055/s-2002-32073 pmid: 12058323
14   Fischer J, Prosenc MH, Wolff M, Hort N, Willumeit R, Feyerabend F. Interference of magnesium corrosion with tetrazolium-based cytotoxicity assays. Acta Biomater 2010;6(5):1813–23
doi: 10.1016/j.actbio.2009.10.020 pmid: 19837191
15   Tominaga H, Ishiyama M, Ohseto F, Sasamoto K, Hamamoto T, Suzuki K, et al. A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Anal Commun 1999;36(2):47–50
doi: 10.1039/a809656b
16   Jia J, Zhao X. GB/T 5009.124–2003 Determination of amino acids in foods. Beijing: Standards Press of China. 2003 Aug 11. Chinese.
17   Wang L, Huang Q, Wang J. Nanostructured polyaniline coating on ITO glass promotes the neurite outgrowth of PC 12 cells by electrical stimulation. Langmuir 2015;31(44):12315–22
doi: 10.1021/acs.langmuir.5b00992 pmid: 25992643
18   ISO 11137-1:2006 Sterilization of health care products—Radiation—Part 1: requirements for development, validation and routine control of a sterilization process for medical devices. Geneva: International Organization for Standardization; 2006 Apr 15.
19   Han Y, Xu Q, Lu Z, Wang J. Preparation of transparent zein films for cell culture applications. Colloid Surface B 2014;120(8):55–62
doi: 10.1016/j.colsurfb.2014.04.019 pmid: 24905679
20   Peng F, Wu H. 620 nm red light promotes celluar viability and mRNA expression of collagen type-I in bone mesenchymal stem cells of rat. In: SOPO 2010: Proceedings of the Symposium on Photonics and Optoelectronic ; 2010 Jun 19–21; Chengdu, China. New Jersey: IEEE; 2010. p. 1–3.
21   Yan T, Sun R, Li C, Tan B, Mao X, Ao N. Immobilization of type-I collagen and basic fibroblast growth factor (bFGF) onto poly (HEMA-co-MMA) hydrogel surface and its cytotoxicity study. J Mater Sci-Mater Med 2010;21(8):2425–33
doi: 10.1007/s10856-010-4094-5 pmid: 20502949
Related
[1] Zhuo Cheng, Lang Qin, Jonathan A. Fan, Liang-Shih Fan. New Insight into the Development of Oxygen Carrier Materials for Chemical Looping Systems[J]. Engineering, 2018, 4(3): 343 -351 .
[2] Jennifer A. Clark, Erik E. Santiso. Carbon Sequestration through CO2 Foam-Enhanced Oil Recovery: A Green Chemistry Perspective[J]. Engineering, 2018, 4(3): 336 -342 .
[3] Andrea Di Maria, Karel Van Acker. Turning Industrial Residues into Resources: An Environmental Impact Assessment of Goethite Valorization[J]. Engineering, 2018, 4(3): 421 -429 .
[4] Lance A. Davis. Falcon Heavy[J]. Engineering, 2018, 4(3): 300 .
[5] Augusta Maria Paci. A Research and Innovation Policy for Sustainable S&T: A Comment on the Essay ‘‘Exploring the Logic and Landscape of the Knowledge System”[J]. Engineering, 2018, 4(3): 306 -308 .
[6] Ning Duan. When Will Speed of Progress in Green Science and Technology Exceed that of Resource Exploitation and Pollutant Generation?[J]. Engineering, 2018, 4(3): 299 .
[7] Jian-guo Li, Kai Zhan. Intelligent Mining Technology for an Underground Metal Mine Based on Unmanned Equipment[J]. Engineering, 2018, 4(3): 381 -391 .
[8] Veena Sahajwalla. Green Processes: Transforming Waste into Valuable Resources[J]. Engineering, 2018, 4(3): 309 -310 .
[9] Junye Wang, Hualin Wang, Yi Fan. Techno-Economic Challenges of Fuel Cell Commercialization[J]. Engineering, 2018, 4(3): 352 -360 .
[10] Raymond RedCorn, Samira Fatemi, Abigail S. Engelberth. Comparing End-Use Potential for Industrial Food-Waste Sources[J]. Engineering, 2018, 4(3): 371 -380 .
[11] Ning Duan, Linhua Jiang, Fuyuan Xu, Ge Zhang. A Non-Contact Original-State Online Real-Time Monitoring Method for Complex Liquids in Industrial Processes[J]. Engineering, 2018, 4(3): 392 -397 .
[12] Keith E. Gubbins, Kai Gu, Liangliang Huang, Yun Long, J. Matthew Mansell, Erik E. Santiso, Kaihang Shi, Małgorzata Ś liwińska-Bartkowiak, Deepti Srivastava. Surface-Driven High-Pressure Processing[J]. Engineering, 2018, 4(3): 311 -320 .
[13] Steff Van Loy, Koen Binnemans, Tom Van Gerven. Mechanochemical-Assisted Leaching of Lamp Phosphors: A Green Engineering Approach for Rare-Earth Recovery[J]. Engineering, 2018, 4(3): 398 -405 .
[14] Robert S. Weber, Johnathan E. Holladay. Modularized Production of Value-Added Products and Fuels from Distributed Waste Carbon-Rich Feedstocks[J]. Engineering, 2018, 4(3): 330 -335 .
[15] Hualin Wang, Pengbo Fu, Jianping Li, Yuan Huang, Ying Zhao, Lai Jiang, Xiangchen Fang, Tao Yang, Zhaohui Huang, Cheng Huang. Separation-and-Recovery Technology for Organic Waste Liquid with a High Concentration of Inorganic Particles[J]. Engineering, 2018, 4(3): 406 -415 .
Copyright © 2015 Higher Education Press & Engineering Sciences Press, All Rights Reserved.
京ICP备11030251号-2

 Engineering