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Engineering    2017, Vol. 3 Issue (1) : 55 -59
Research |
Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite
Alessandro Pistone1(),Daniela Iannazzo1,Claudia Espro1,Signorino Galvagno1,Anna Tampieri2,Monica Montesi2,Silvia Panseri2,Monica Sandri2
1. Department of Engineering, University of Messina, Messina 98166, Italy
2. Institute of Science and Technology for Ceramics, National Research Council of Italy, Faenza 48018, Italy

Stem cell homing, namely the recruitment of mesenchymal stem cells (MSCs) to injured tissues, is highly effective for bone regeneration in vivo. In order to explore whether the incorporation of mimetic peptide sequences on magnesium-doped (Mg-doped) hydroxyapatite (HA) may regulate the homing of MSCs, and thus induce cell migration to a specific site, we covalently functionalized MgHA disks with two chemotactic/haptotactic factors: either the fibronectin fragment III1-C human (FF III1-C), or the peptide sequence Gly-Arg-Gly-Asp-Ser-Pro-Lys, a fibronectin analog that is able to bind to integrin transmembrane receptors. Preliminary biological evaluation of MSC viability, analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, suggested that stem cells migrate to the MgHA disks in response to the grafted haptotaxis stimuli.

Keywords Mg-doped hydroxyapatite      Mesenchymal stem cells      Chemotactic/haptotactic factors      Bone tissue engineering     
Corresponding Authors: Alessandro Pistone   
Just Accepted Date: 20 February 2017   Online First Date: 27 February 2017    Issue Date: 02 March 2017
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Alessandro Pistone
Daniela Iannazzo
Claudia Espro
Signorino Galvagno
Anna Tampieri
Monica Montesi
Silvia Panseri
Monica Sandri
Cite this article:   
Alessandro Pistone,Daniela Iannazzo,Claudia Espro, et al. Tethering of Gly-Arg-Gly-Asp-Ser-Pro-Lys Peptides on Mg-Doped Hydroxyapatite[J]. Engineering, 2017, 3(1): 55 -59 .
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