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Engineering    2017, Vol. 3 Issue (1) : 36 -54
Research |
Biophysical Regulation of Cell Behavior—Cross Talk between Substrate Stiffness and Nanotopography
Yong Yang1(),Kai Wang1,Xiaosong Gu2,Kam W. Leong3()
1. Department of Chemical and Biomedical Engineering, West Virginia University, Morgantown, WV 26506, USA
2. Key Laboratory of Neuroregeneration of Jiangsu and the Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China
3. Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA

The stiffness and nanotopographical characteristics of the extracellular matrix (ECM) influence numerous developmental, physiological, and pathological processes in vivo. These biophysical cues have therefore been applied to modulate almost all aspects of cell behavior, from cell adhesion and spreading to proliferation and differentiation. Delineation of the biophysical modulation of cell behavior is critical to the rational design of new biomaterials, implants, and medical devices. The effects of stiffness and topographical cues on cell behavior have previously been reviewed, respectively; however, the interwoven effects of stiffness and nanotopographical cues on cell behavior have not been well described, despite similarities in phenotypic manifestations. Herein, we first review the effects of substrate stiffness and nanotopography on cell behavior, and then focus on intracellular transmission of the biophysical signals from integrins to nucleus. Attempts are made to connect extracellular regulation of cell behavior with the biophysical cues. We then discuss the challenges in dissecting the biophysical regulation of cell behavior and in translating the mechanistic understanding of these cues to tissue engineering and regenerative medicine.

Keywords Extracellular matrix      Stiffness      Nanotopography      Adhesive ligands      Cell behavior     
Corresponding Authors: Yong Yang,Kam W. Leong   
Just Accepted Date: 21 February 2017   Online First Date: 28 February 2017    Issue Date: 02 March 2017
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Yong Yang,Kai Wang,Xiaosong Gu, et al. Biophysical Regulation of Cell Behavior—Cross Talk between Substrate Stiffness and Nanotopography[J]. Engineering, 2017, 3(1): 36 -54 .
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