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Engineering    2015, Vol. 1 Issue (2) : 269 -274
Research |
Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids
Kai Ling1,2,Guoyou Huang2,3,Juncong Liu2,Xiaohui Zhang2,3,Yufei Ma2,3,Tianjian Lu2,(),Feng Xu2,3,()
1. State Key Laboratory for Strength and Vibration of Mechanical Structures, School of Aerospace, Xi’an Jiaotong University, Xi’an 710049, China
2. Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an 710049, China
3. The Key Laboratory of Biomedical Information Engineering of the Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China

Cellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cell-embedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.

Keywords MCF-7 cellular spheroids      bioprinting      hydrogels      concave wells      tissue on a chip     
Corresponding Authors: Tianjian Lu,Feng Xu   
Just Accepted Date: 30 June 2015   Issue Date: 16 September 2015
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Kai Ling
Guoyou Huang
Juncong Liu
Xiaohui Zhang
Yufei Ma
Tianjian Lu
Feng Xu
Cite this article:   
Kai Ling,Guoyou Huang,Juncong Liu, et al. Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids[J]. Engineering, 2015, 1(2): 269 -274 .
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