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Generation and repair of AID-initiated DNA lesions in B lymphocytes

Zhangguo Chen,Jing H. Wang

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 201-216 doi: 10.1007/s11684-014-0324-4

Abstract: In mammalian B cells, this process includes somatic hypermutation (SHM) and class switch recombinationreview, we focus on the molecular mechanisms that regulate the specificity of AID targeting and the repair

Keywords： class switch recombination     somatic hypermutation     activation-induced deaminase     DNA repair     genomic instability    

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Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 67-71 doi: 10.1007/s11684-009-0010-0

Abstract: Divalent metal transporter 1 (DMT1) is a ferrous iron import protein. The improper expression of DMT1 is involved in neurodegenerative diseases. In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without the iron response element (DMT1-IRE) and investigated its expression and function in the C6 glioma cell line. The DMT1-IRE gene, obtained by RT-PCR, was cloned into the shuttle plasmid pAdTrack-CMV containing green fluorescent protein (GFP) reporter gene. Linearized plasmid pAdTrack-CMV-DMT1-IRE was subsequently co-transformed into ( ) BJ5183 cells along with an adenoviral backbone plasmid pAdEasy-1 after digestion with I. I-digested pAdEasy1-DMT1-IRE was then transfected into E1-transformed human embryonic kidney cells (HEK293 cells) , in which recombinant adenoviruses were generated within 7 to 10 days. The results demonstrated that we obtained the DMT1-IRE gene. pAdEasy1-DMT1-IRE yielded a large fragment, plus a smaller fragment of 4.5 kb after digestion with I. PCR confirmed pAdEasy1-DMT1-IRE contained gene DMT1-IRE, indicating the successful construction of recombinant adenovirus plasmid containing DMT1-IRE. GFP fluorescence further confirmed the generation of adenovirus. AdDMT1-IRE could efficiently infect C6 glioma cells. And cell viability decreased in Ad-DMT1-IRE infected cells after iron overload compared to the control. These results suggest that the over expressed DMT1-IRE can aggravate the iron induced cell death due to its iron influx function.

Keywords： divalent metal transporter 1     recombinant adenovirus     homologous recombination     iron    

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Detecting genetic hypermutability of gastrointestinal tumor by using a forensic STR kit

Anqi Chen, Suhua Zhang, Jixi Li, Chaoneng Ji, Jinzhong Chen, Chengtao Li

Frontiers of Medicine 2020, Volume 14, Issue 1,   Pages 101-111 doi: 10.1007/s11684-019-0698-4

Abstract: Growing evidence suggests that somatic hypermutational status and programmed cell death-1 overexpression are potential predictive biomarkers indicating treatment benefits from immunotherapy using immune checkpoint inhibitors. However, biomarker-matched trials are still limited, and many of the genomic alterations remain difficult to target. To isolate the potential somatic hypermutational tumor from microsatellite instability low/microsatellite stability (MSI-L/MSS) cases, we employed two commercial kits to determine MSI and forensic short tandem repeat (STR) alternations in 250 gastrointestinal (GI) tumors. Three types of forensic STR alternations, namely, allelic loss, Aadd, and Anew, were identified. 62.4% (156/250) of the patients with GI exhibited STR alternation, including 100% (15/15) and 60% (141/235) of the microsatellite high instability and MSI-L/MSS cases, respectively. 30% (75/250) of the patients exhibited STR instability with more than 26.32% (26.32%–84.21%) STR alternation. The cutoff with 26.32% of the STR alternations covered all 15 MSI cases and suggested that it might be a potential threshold. Given the similar mechanism of the mutations of MSI and forensic STR, the widely used forensic identifier STR kit might provide potential usage for identifying hypermutational status in GI cancers.

Keywords： mismatch repair protein deficiency (MMR-D)     microsatellite instability (MSI)     short tandem repeats (STR    

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Antithrombin deficiency and decreased protein C activity in a young man with venous thromboembolism:

Dong Wang, Min Tian, Guanglin Cui, Dao Wen Wang

Frontiers of Medicine 2018, Volume 12, Issue 3,   Pages 319-323 doi: 10.1007/s11684-017-0553-4

Abstract: Mutations in and lead to deficiency or dysfunction of the two proteins, which could result in venousCoagulation tests revealed that the patient presented type I antithrombin deficiency combined with decreasedThis study demonstrates that the combination of antithrombin deficiency and decreased protein C activity

Keywords： antithrombin deficiency     protein C activity     mutation     variant     venous thromboembolism     anticoagulants    

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Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice

Frontiers of Medicine 2023, Volume 17, Issue 5,   Pages 957-971 doi: 10.1007/s11684-023-0988-8

Abstract: To the best of our knowledge, this study is the first to report DNAH10 deficiency related to PCD

Keywords： DNAH10     mice     motile cilia     mutation     primary ciliary dyskinesia    

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Effects of BTA2 as the third component on the charge carrier generation and recombination behavior of

Leijing Liu, Hao Zhang, Bo Xiao, Yang Liu, Bin Xu, Chen Wang, Shanpeng Wen, Erjun Zhou, Gang Chen, Chan Im, Wenjing Tian

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 1,   Pages 127-137 doi: 10.1007/s11705-020-1936-7

Abstract: benzotriazole (BTA)-based small molecule, BTA2, as the third component on the charge carrier generation and recombinationphoto-CELIV investigation indicates that the ternary OSCs suffer higher charge trap-limited bimolecular recombination

Keywords： third component     organic solar cells     charge carrier generation     charge carrier recombination     bimolecularrecombination    

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Comprehensive functional annotation of susceptibility variants identifies genetic heterogeneity between lung adenocarcinoma and squamous cell carcinoma

Na Qin, Yuancheng Li, Cheng Wang, Meng Zhu, Juncheng Dai, Tongtong Hong, Demetrius Albanes, Stephen Lam, Adonina Tardon, Chu Chen, Gary Goodman, Stig E. Bojesen, Maria Teresa Landi, Mattias Johansson, Angela Risch, H-Erich Wichmann, Heike Bickeboller, Gadi Rennert, Susanne Arnold, Paul Brennan, John K. Field, Sanjay Shete, Loic Le Marchand, Olle Melander, Hans Brunnstrom, Geoffrey Liu, Rayjean J. Hung, Angeline Andrew, Lambertus A. Kiemeney, Shan Zienolddiny, Kjell Grankvist, Mikael Johansson, Neil Caporaso, Penella Woll, Philip Lazarus, Matthew B. Schabath, Melinda C. Aldrich, Victoria L. Stevens, Guangfu Jin, David C. Christiani, Zhibin Hu, Christopher I. Amos, Hongxia Ma, Hongbing Shen

Frontiers of Medicine 2021, Volume 15, Issue 2,   Pages 275-291 doi: 10.1007/s11684-020-0779-4

Abstract: Analysis revealed that AD genes were primarily involved in immune-related pathways, while SqCC genes were homologousrecombination deficiency related.

Keywords： lung cancer     genome-wide association study     function annotation     immune     homologous recombination repairdeficiency     genetic heterogeneity    

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Prevalence of vitamin D deficiency in girls with idiopathic central precocious puberty

Yue Zhao, Wenjun Long, Caiqi Du, Huanhuan Yang, Shimin Wu, Qin Ning, Xiaoping Luo

Frontiers of Medicine 2018, Volume 12, Issue 2,   Pages 174-181 doi: 10.1007/s11684-017-0544-5

Abstract:

The relationship between vitamin D deficiency and idiopathic central precocious puberty (ICPP) hasThe ICPP subjects with 25[OH]D deficiency had a higher body mass index (BMI) than the subjects from the

Keywords： idiopathic central precocious puberty     threshold effects     vitamin D status    

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A distinct “repair” role of regulatory T cells in fracture healing

Frontiers of Medicine 2024, Volume 18, Issue 3,   Pages 516-537 doi: 10.1007/s11684-023-1024-8

Abstract: Regulatory T cells (Tregs) suppress immune responses and inflammation. Here, we described the distinct nonimmunological role of Tregs in fracture healing. The recruitment from the circulation pool, peripheral induction, and local expansion rapidly enriched Tregs in the injured bone. The Tregs in the injured bone displayed superiority in direct osteogenesis over Tregs from lymphoid organs. Punctual depletion of Tregs compromised the fracture healing process, which leads to increased bone nonunion. In addition, bone callus Tregs showed unique T-cell receptor repertoires. Amphiregulin was the most overexpressed protein in bone callus Tregs, and it can directly facilitate the proliferation and differentiation of osteogenic precursor cells by activation of phosphatidylinositol 3-kinase/protein kinase B signaling pathways. The results of loss- and gain-function studies further evidenced that amphiregulin can reverse the compromised healing caused by Treg dysfunction. Tregs also enriched in patient bone callus and amphiregulin can promote the osteogenesis of human pre-osteoblastic cells. Our findings indicate the distinct and nonredundant role of Tregs in fracture healing, which will provide a new therapeutic target and strategy in the clinical treatment of fractures.

Keywords： regulatory T cells     fracture healing     amphiregulin     non-union     osteogenesis    

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Bile duct injury repair — earlier is not better

Vinay K. Kapoor

Frontiers of Medicine 2015, Volume 9, Issue 4,   Pages 508-511 doi: 10.1007/s11684-015-0418-7

Abstract: The timing of bile duct injury repair remains controversial.A recent review conducted in France reported 39% complications and 64% failure after immediate repairin 194 patients compared with 14% complications and 8% failure after late repair in 133 patients.Most patients with bile duct injury present with bile leak and sepsis; thus, early repair is not recommended

Keywords： bile duct injury     cholecystectomy     laparoscopic cholecystectomy    

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non-inferiority trial of intravenous ferric carboxymaltose versus iron sucrose in patients with iron deficiency

Frontiers of Medicine 2024, Volume 18, Issue 1,   Pages 98-108 doi: 10.1007/s11684-023-1001-2

Abstract: Iron deficiency (ID) and ID anemia (IDA) pose significant public health concerns in China.

Keywords： iron deficiency     anemia     intravenous iron     ferric carboxymaltose     iron sucrose     Hb response     early response    

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Expression status of GATA3 and mismatch repair proteins in upper tract urothelial carcinoma

Yue Wang, Jinxia Zhang, Yunfan Wang, Shufang Wang, Yu Zhang, Qi Miao, Fei Gao, Huiying He

Frontiers of Medicine 2019, Volume 13, Issue 6,   Pages 730-740 doi: 10.1007/s11684-019-0687-7

Abstract: GATA binding protein 3 (GATA3) and mismatch repair (MMR) deficiency contribute to the development of

Keywords： upper tract urothelial carcinoma     GATA binding protein 3     mismatch repair     microsatellite instability     prognosis    

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Experimental study and field application of calcium sulfoaluminate cement for rapid repair of concrete

Yanhua GUAN, Ying GAO, Renjuan SUN, Moon C. WON, Zhi GE

Frontiers of Structural and Civil Engineering 2017, Volume 11, Issue 3,   Pages 338-345 doi: 10.1007/s11709-017-0411-0

Abstract: The fast-track repair of deteriorated concrete pavement requires materials that can be placed, cured,Based on the laboratory results, the CSA cement was selected as the partial-depth rapid repair materialThe data collected during and after the repair show that the CSA cement concrete had good short-termand long-term performances and, therefore, was suitable for the rapid repair of concrete pavement.

Keywords： Sulfoaluminate (CSA) cement     Type III cement     coefficient of thermal expansion (CTE)     shrinkage     rapid repair    

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Mobile platform for hydraulic turbine blade repair robot

GUI Zhongcheng, CHEN Qiang, SUN Zhenguo, ZHANG Wenzeng, LIU Kang

Frontiers of Mechanical Engineering 2008, Volume 3, Issue 2,   Pages 164-169 doi: 10.1007/s11465-008-0035-0

Abstract: adapt to complex spatial surfaces, which satisfy the requirements of a hydraulic turbine blade in-situ repair
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Research on concrete structure defect repair based on three-dimensional printing

Frontiers of Structural and Civil Engineering 2024, Volume 18, Issue 5,   Pages 731-742 doi: 10.1007/s11709-024-1088-9

Abstract: computer vision-based robotic system, which is based on three-dimensional (3D) printing technology to repairFinally, the feasibility of this concrete defect repair method is validated through simulation and experiments

Keywords： concrete     defect detection     3D printing     deep learning     point cloud data    

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Title Author Date Type Operation

Generation and repair of AID-initiated DNA lesions in B lymphocytes

Zhangguo Chen,Jing H. Wang

Journal Article

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Journal Article

Detecting genetic hypermutability of gastrointestinal tumor by using a forensic STR kit

Anqi Chen, Suhua Zhang, Jixi Li, Chaoneng Ji, Jinzhong Chen, Chengtao Li

Journal Article

Antithrombin deficiency and decreased protein C activity in a young man with venous thromboembolism:

Dong Wang, Min Tian, Guanglin Cui, Dao Wen Wang

Journal Article

Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice

Journal Article

Effects of BTA2 as the third component on the charge carrier generation and recombination behavior of

Leijing Liu, Hao Zhang, Bo Xiao, Yang Liu, Bin Xu, Chen Wang, Shanpeng Wen, Erjun Zhou, Gang Chen, Chan Im, Wenjing Tian

Journal Article

Comprehensive functional annotation of susceptibility variants identifies genetic heterogeneity between lung adenocarcinoma and squamous cell carcinoma

Na Qin, Yuancheng Li, Cheng Wang, Meng Zhu, Juncheng Dai, Tongtong Hong, Demetrius Albanes, Stephen Lam, Adonina Tardon, Chu Chen, Gary Goodman, Stig E. Bojesen, Maria Teresa Landi, Mattias Johansson, Angela Risch, H-Erich Wichmann, Heike Bickeboller, Gadi Rennert, Susanne Arnold, Paul Brennan, John K. Field, Sanjay Shete, Loic Le Marchand, Olle Melander, Hans Brunnstrom, Geoffrey Liu, Rayjean J. Hung, Angeline Andrew, Lambertus A. Kiemeney, Shan Zienolddiny, Kjell Grankvist, Mikael Johansson, Neil Caporaso, Penella Woll, Philip Lazarus, Matthew B. Schabath, Melinda C. Aldrich, Victoria L. Stevens, Guangfu Jin, David C. Christiani, Zhibin Hu, Christopher I. Amos, Hongxia Ma, Hongbing Shen

Journal Article

Prevalence of vitamin D deficiency in girls with idiopathic central precocious puberty

Yue Zhao, Wenjun Long, Caiqi Du, Huanhuan Yang, Shimin Wu, Qin Ning, Xiaoping Luo

Journal Article

A distinct “repair” role of regulatory T cells in fracture healing

Journal Article

Bile duct injury repair — earlier is not better

Vinay K. Kapoor

Journal Article

non-inferiority trial of intravenous ferric carboxymaltose versus iron sucrose in patients with iron deficiency

Journal Article

Expression status of GATA3 and mismatch repair proteins in upper tract urothelial carcinoma

Yue Wang, Jinxia Zhang, Yunfan Wang, Shufang Wang, Yu Zhang, Qi Miao, Fei Gao, Huiying He

Journal Article

Experimental study and field application of calcium sulfoaluminate cement for rapid repair of concrete

Yanhua GUAN, Ying GAO, Renjuan SUN, Moon C. WON, Zhi GE

Journal Article

Mobile platform for hydraulic turbine blade repair robot

GUI Zhongcheng, CHEN Qiang, SUN Zhenguo, ZHANG Wenzeng, LIU Kang

Journal Article

Research on concrete structure defect repair based on three-dimensional printing

Journal Article